RESEARCH NETWORK FOR NEW STRATEGIES IN IMMUNOTHERAPY
D5 - Recombinant Polypeptides with Optimized T-Cell Activating Properties
Recombinantly produced proteins are frequently used to stimulate T-cell responses either in diagnostic (e.g. detection of virus-specific T-cells) or immunoprophylaxis settings (i.e. as a vaccine). The pathogen-derived proteins, however, differ in their stimulatory capacity due to diverse inherent protein properties. The immediate early 1 (IE1) protein of cytomegalovirus (CMV) would be an interesting candidate for the above named applications due to its high and early expression during the viral replication cycle, however it lacks potent stimulatory activity. Therefore, our aim in this project is to modify and formulate the protein in a manner to increase the T-cell-activating properties. The resulting IE1-variants/formulations will be tested in suitable ex vivo and in vivo models for their potential to re-stimulate or prime naïve IE1-specific T-cells.